| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Steckiel, Emma J (13)
|
MAUN06 |
|
2:21.08 |
- |
| 1 |
Franciscus, Kimberly A (14)
|
MAJY |
2:15.93 |
2:21.96 |
6.03 |
| 1 |
Gally, Annika L (13)
|
MAAAC |
2:27.65 |
2:23.82 |
-3.83 |
| 2 |
Osanitsch, Madison E (13)
|
MASHIP |
|
2:23.90 |
- |
| 2 |
Snyder, Savanah M (13)
|
MAAAC |
2:29.47 |
2:29.85 |
0.38 |
| 3 |
Bradburn, Amalia H (13)
|
MAAAC |
|
2:28.26 |
- |
| 3 |
Burkhardt, Carey S (13)
|
MAAAC |
2:29.17 |
2:32.89 |
3.72 |
| 4 |
Burke, Alexandra R (14)
|
MAJY |
2:32.40 |
2:37.69 |
5.29 |
| 4 |
Turner, Peyton A (13)
|
MAACST |
|
2:44.82 |
- |
| 5 |
Hurley, Colby L (13)
|
MATVAC |
|
3:04.83 |
- |
| 6 |
DeTrempe, Gabrielle E (13)
|
MANHAC |
|
3:24.80 |
- |